Another Mode of Action of Temephos Against Aedes aegypti Larvae: A Stomach Poison Investigation

Introduction: Aedes aegypti is a key vector for the spread of several severe arboviral infections. The Indonesian Ministry Health launched Temephos as national effort to limit larvae population. old theory has been passed down generations that principle mechanism action temephos neurotoxin. main aim this study was investigate stomach poison by using histopathology study. Method: There are two treatments with three replications: container containing only 100 ml water tween 20 and 1 ppm 8G. third-instar Ae. in containers 8G were compared those Tween-20. experiment done replications. number dead recorded after 24 hours treatment. Histological sections larval midgut prepared stained hematoxylin-eosin (HE). Light microscopy used examine changes length lumen epithelium. Data analyzed one-way ANOVA. appearances nucleus epithelial cell degree damage qualitatively observed. Results: results showed no found control group, however, 100% mortality group. epithelium significantly different from group (p<0.05). Nuclei cells lost damaged Conclusions: This reports first discovery other than neurotoxin, namely